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KMID : 0914820050050040260
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Journal of the Korean Gastric Cancer Association
2005 Volume.5 No. 4 p.260 ~ p.265
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Mutation of the Chk1 Gene in Gastric Cancers with Microsatellite Instability
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Lee Jong-Heun
Cho Yong-Gu
Song Jae-Hwi
Park Cho-Hyun
Kim Su-Young
Nam Suk-Woo
Lee Sug-Hyung
Yoo Nam-Jin
Lee Jung-Yong
Park Won-Sang
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Abstract
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Purpose: The protein kinase Chk1 is required for cell cycle arrest in response to DNA damage and is shown to play an important role in the G2/M checkpoint. The aim of this study was to investigate the relationship between microsatellite instability and frameshift mutation of the Chk1 gene in gastric cancers.
Materials and Methods: The microsatellite instability was analyzed in 95 primary gastric carcinomas by using microdissection and 6 microsatellite markers. We also performed single strand conformational polymorphism and sequencing to detect frameshift mutation of the Chk1 gene.
Results: We found positive microsatellite instability in 19 (20%) of the 95 gastric cancers, 13 high- and 6 low-frequency microsatellite instability cases. The frameshift mutation of Chk1, which resulted in a truncated Chk1 protein, was detected in two high-frequency microsatellite instability cases.
Conclusion: These data suggest that the microsatellite instability may contribute to the development of gastric carcinomas through inactivation of Chk1.
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KEYWORD
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Gastric cancer, Microsatellite istability, Mutation, Cell cycle
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